Modulation of neuronal cell affinity on PEDOT–PSS non-woven silk scaffolds for neural tissue engineering

Magaz, Adrián and Spencer, Ben and Hardy, John and Li, Xu and Gough, Julie and Blaker, Jonny (2020) Modulation of neuronal cell affinity on PEDOT–PSS non-woven silk scaffolds for neural tissue engineering. ACS Biomaterials Science and Engineering, 6 (12). 6906–6916. ISSN 2373-9878

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Abstract

Peripheral nerve injury is a common consequence of trauma with low regenerative potential. Electroconductive scaffolds can provide appropriate cell growth microenvironments and synergistic cell guidance cues for nerve tissue engineering. In the present study, electrically conductive scaffolds were prepared by conjugating poly(3,4-ethylenedioxythiophene)-polystyrene sulfonate (PEDOT–PSS) or dimethyl sulfoxide (DMSO)-treated PEDOT–PSS on electrospun silk scaffolds. Conductance could be tuned by the coating concentration and was further boosted by DMSO treatment. Analogue NG108-15 neuronal cells were cultured on the scaffolds to evaluate neuronal cell growth, proliferation and differentiation. Cellular viability was maintained on all scaffold groups, while showing comparatively better metabolic activity and proliferation than unmodified silk. DMSO-treated PEDOT–PSS functionalized scaffolds partially outperformed their PEDOT–PSS counterparts. Differentiation assessments suggested that these PEDOT–PSS assembled silk scaffolds could support neurite sprouting, indicating that they show promise to be used as a future platform to restore electrochemical coupling at the site of injury and preserve normal nerve function.

Item Type:
Journal Article
Journal or Publication Title:
ACS Biomaterials Science and Engineering
Additional Information:
This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Biomaterials Science & Engineering, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/doi/10.1021/acsbiomaterials.0c01239
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/2500/2502
Subjects:
ID Code:
148262
Deposited By:
Deposited On:
16 Oct 2020 08:20
Refereed?:
Yes
Published?:
Published
Last Modified:
17 Feb 2021 09:02