Sensitive morphological characterization of oriented high‐density lipoprotein nanoparticles using 31P NMR spectroscopy

Lau, Sophie and Middleton, David (2020) Sensitive morphological characterization of oriented high‐density lipoprotein nanoparticles using 31P NMR spectroscopy. Angewandte Chemie International Edition. ISSN 1433-7851

Full text not available from this repository.

Abstract

The biological function of high‐density lipoprotein (HDL) nanoparticles, the so‐called good cholesterol that is associated with a low risk of heart disease, depends on their composition, morphology and size. The morphology of HDL particles composed of apolipoproteins, lipids and cholesterol is routinely visualised by transmission electron microscopy (TEM), but higher‐resolution tools are needed to observe more subtle structural differences between particles of different composition. Here, reconstituted HDL formulations are oriented on glass substrates and solid‐state 31 P NMR spectroscopy is shown to be highly sensitive to the surface curvature of the lipid headgroups. The spectra report potentially functionally important differences in the morphology of different HDL preparations that are not detected by TEM. This method provides new morphological insights into HDL comprising a naturally‐occurring apolipoprotein A‐I mutant, which may be linked to its atheroprotective properties, and holds promise as a future research tool in the clinical analysis of plasma HDL.

Item Type:
Journal Article
Journal or Publication Title:
Angewandte Chemie International Edition
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1500/1503
Subjects:
ID Code:
144767
Deposited By:
Deposited On:
16 Jun 2020 08:45
Refereed?:
Yes
Published?:
Published
Last Modified:
27 Sep 2020 05:51