A method for the permeabilization of live Drosophila melanogaster larvae to small molecules and cryoprotectants

Murray, A. and Palmer, D. and Bennett, D. and Dwarampudi, V. and Pedro de Magalhães, J. (2020) A method for the permeabilization of live Drosophila melanogaster larvae to small molecules and cryoprotectants. Fly.

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Abstract

The larvae of Drosophila melanogaster is a model organism widely used to study the muscular and nervous systems. Drosophila larvae are surrounded by a waxy cuticle that prevents permeation by most substances. Here we develop a method to remove this layer, rendering the larvae permeable to small molecules without causing death, allowing the larvae to develop to adulthood and reproduce. Permeability was assessed using fluorescein diacetate dye uptake, and mortality upon exposure to toxic levels of ethylene glycol (EG) and Dimethyl sulfoxide (DMSO). Potential uses for this method include drug delivery, toxicity assays, cryopreservation, staining, and fixation.

Item Type:
Journal Article
Journal or Publication Title:
Fly
Additional Information:
Export Date: 20 February 2020
ID Code:
141662
Deposited By:
Deposited On:
24 Feb 2020 16:10
Refereed?:
Yes
Published?:
Published
Last Modified:
29 Mar 2020 07:00