Stress responses and specific metal exclusion on mine soils based on germination and growth studies by Australian golden wattle

Nirola, Ramkrishna and Megharaj, Mallavarapu and Aryal, Rupak and Thavamani, Palanisami and Ramdass, Kavitha and Sarkar, Binoy and Saint, Christopher (2016) Stress responses and specific metal exclusion on mine soils based on germination and growth studies by Australian golden wattle. Ecological Indicators, 71. pp. 113-122. ISSN 1470-160X

Abstract

We reported the Australian golden wattle as a copper stabilizer in abandoned copper mine soils earlier. Here we investigate to confirm this plant’s suitability to grow on metal contaminated mine soils based on stress indication. The seeds of Acacia pycnantha collected from mining area were germinated after heat and no heat treatment on two types of irrigation. The daily irrigated and heat treated seeds gave up to 85% germination on sandy soil. The A. pycnantha was grown under greenhouse condition in six different soils collected from abandoned copper mine at Kapunda in South Australia. Among the six soil samples, soil-1 with the highest copper concentration produced 2.05 mmol g−1 tissue of proline. Proline expression was prominent in more saline soils (1, 5 and 6) having electrical conductivity (EC) 1184, 1364 and 1256 μS, respectively. Chlorophyll a, b and carotenoid levels in plants showed a gradually decreasing trend in all the soils as experiment progressed. The plants grown on soil sample-1, containing 4083 ± 103 mg kg−1 of copper resulted in 18 ± 2 mg kg−1 accumulation in its leaf. The calcium accumulation was significant up to 11648 ± 1209 mg kg−1 in leaf. Although pore water samples showed higher Cu concentration in soils, an increased mobility of arsenic and lead was observed in all the soil samples. Our experiment points out the need for proper monitoring of revegetation processes to avoid revegetation and reclamation failure.