Visceral leishmaniasis in Teresina, n. e. Brazil:towards a DNA probe kit and its adaptation to processing blood-contaminated samples.

McNerney, R. and Frame, I. A. and Vexenat, J. A. and Fonseca de Castro, J. A. and Howard, M. K. and Dillon, R. and Miles, M. A. (1993) Visceral leishmaniasis in Teresina, n. e. Brazil:towards a DNA probe kit and its adaptation to processing blood-contaminated samples. Archives de l'Institut Pasteur de Tunis, 70 (3-4). pp. 405-418. ISSN 0020-2509

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Abstract

The Lmet2 chemiluminescent DNA probe is a valuable tool for identifying parasites of the Leishmania donovani -complex in sand flies, dogs and human samples. Recent blood meals in sand flies or blood contamination of tissue samples inhibited probe sensitivity, whether radiolabelled or chemiluminescent detection systems were used. Treatment of membranes with protease before hybridisation restored positive signal. Alternatively samples could be lysed with protease and applied to membranes with a vacuum blotting apparatus. The Lmet2 protocol provides the basis for a DNA probe kit that is adaptable for use with a wide range of other probes.

Item Type:
Journal Article
Journal or Publication Title:
Archives de l'Institut Pasteur de Tunis
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/2700
Subjects:
ID Code:
139485
Deposited By:
Deposited On:
09 Dec 2019 09:25
Refereed?:
Yes
Published?:
Published
Last Modified:
01 Jan 2020 12:22