Delivery and quantification of hydrogen peroxide generated via cold atmospheric pressure plasma through biological material

Hathaway, H.J. and Patenall, B.L. and Thet, N.T. and Sedgwick, A.C. and Williams, G.T. and Jenkins, A.T.A. and Allinson, S.L. and Short, R.D. (2019) Delivery and quantification of hydrogen peroxide generated via cold atmospheric pressure plasma through biological material. Journal of Physics D: Applied Physics, 52 (50). ISSN 0022-3727

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Abstract

The ability of plasma-generated hydrogen peroxide (H 2O 2) to traverse bacterial biofilms and the subsequent fate of the generated H 2O 2 has been investigated. An in vitro model, comprising a nanoporous membrane impregnated with artificial wound fluid and biofilms of varying maturity was treated with a helium-driven, cold atmospheric pressure plasma (CAP) jet. The concentration of H 2O 2 generated below the biofilms was quantified. The results showed that the plasma-generated H 2O 2 interacted significantly with the biofilm, thus exhibiting a reduction in concentration across the underlying nanoporous membrane. Biofilm maturity exhibited a significant effect on the penetration depth of H 2O 2, suggesting that well established, multilayer biofilms are likely to offer a shielding effect with respect to cells located in the lower layers of the biofilm, thus rendering them less susceptible to plasma disinfection. This may prove clinically significant in the plasma treatment of chronic, deep tissue infections such as diabetic and venous leg ulcers. Our results are discussed in the context of plasma-biofilm interactions, with respect to the fate of the longer lived reactive species generated by CAP, such as H 2O 2

Item Type: Journal Article
Journal or Publication Title: Journal of Physics D: Applied Physics
Additional Information: This is an author-created, un-copyedited version of an article accepted for publication/published in Journal of Physics D: Applied Physics. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at doi: 10.1088/1361-6463/ab4539
Uncontrolled Keywords: /dk/atira/pure/subjectarea/asjc/3100/3104
Subjects:
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 138444
Deposited By: ep_importer_pure
Deposited On: 05 Nov 2019 10:15
Refereed?: Yes
Published?: Published
Last Modified: 20 Feb 2020 04:24
URI: https://eprints.lancs.ac.uk/id/eprint/138444

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