Characterization and expression of an mRNA encoding a wound-induced (win) protein from ethylene-treated tomato leaf abscission zone tissue

Harris, Neil and Taylor, Jane E. and Roberts, Jeremy A. (1997) Characterization and expression of an mRNA encoding a wound-induced (win) protein from ethylene-treated tomato leaf abscission zone tissue. Journal of Experimental Botany, 48 (6). pp. 1223-1227. ISSN 0022-0957

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Abstract

A cDNA clone (TAB7) encoding a putative wound-induced (Win) protein has been isolated from a tomato (Lycopersicon esculentum Mill. cv. Ailsa Craig) leaf abscission zone cDNA library using a differential screening strategy. The clone has a high degree of homology at the amino acid level to both the potato win1 and 2 genes, Hevea brasiliensis hevein and Nicotiana tabacum PR-4a and PR-4b proteins. The mRNA encoded by TAB7 is up-regulated within 12 h of exposure to ethylene (10 μl l-1) and its expression increases steadily within the cells comprising the leaf abscission zone and to a lesser extent in the adjacent non-zone tissue. This rise precedes the onset of cell separation. Southern analysis indicates that the mRNA is encoded by either a single gene or a small gene family. The role of the protein during abscission is discussed.

Item Type:
Journal Article
Journal or Publication Title:
Journal of Experimental Botany
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1100/1110
Subjects:
ID Code:
137879
Deposited By:
Deposited On:
14 Oct 2019 14:06
Refereed?:
Yes
Published?:
Published
Last Modified:
01 Jan 2020 12:18