Role of Schizosaccharomyces pombe RecQ homolog, recombination, and checkpoint genes in UV damage tolerance

Murray, Johanne M. and Lindsay, Howard D. and Munday, Caroline A. and Carr, Antony M. (1997) Role of Schizosaccharomyces pombe RecQ homolog, recombination, and checkpoint genes in UV damage tolerance. Molecular and Cellular Biology, 17 (12). pp. 6868-6875. ISSN 0270-7306

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Abstract

The cellular responses to DNA damage are complex and include direct DNA repair pathways that remove the damage and indirect damage responses which allow cells to survive DNA damage that has not been, or cannot be, removed. We have identified the gene mutated in the rad12.502 strain as a Schizosaccharomyces pombe recQ homolog. The same gene (designated rqh1) is also mutated in the hus2.22 mutant. We show that Rqh1 is involved in a DNA damage survival mechanism which prevents cell death when UV-induced DNA damage cannot be removed. This pathway also requires the correct functioning of the recombination machinery and the six checkpoint tad gene products plus the Cds1 kinase. Our data suggest that Rqh1 operates during S phase as part of a mechanism which prevents DNA damage causing cell lethality. This process may involve the bypass of DNA damage sites by the replication fork. Finally, in contrast with the reported literature, we do not find that rqh1 (rad12) mutant cells are defective in UV dimer endonuclease activity.

Item Type: Journal Article
Journal or Publication Title: Molecular and Cellular Biology
Uncontrolled Keywords: /dk/atira/pure/subjectarea/asjc/1300/1307
Subjects:
Departments: Faculty of Health and Medicine > Medicine
ID Code: 137770
Deposited By: ep_importer_pure
Deposited On: 11 Oct 2019 13:15
Refereed?: Yes
Published?: Published
Last Modified: 18 Nov 2019 04:17
URI: https://eprints.lancs.ac.uk/id/eprint/137770

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