Development of Leishmania orientalis in the sand fly Lutzomyia longipalpis (Diptera: Psychodidae) and the biting midge Culicoides soronensis (Diptera: Ceratopogonidae)

Chanmol, Wetpisit and Jariyapan, Narissara and Somboon, Pradya and Bates, Michelle D and Bates, Paul A (2019) Development of Leishmania orientalis in the sand fly Lutzomyia longipalpis (Diptera: Psychodidae) and the biting midge Culicoides soronensis (Diptera: Ceratopogonidae). Acta Tropica, 199: 105157. ISSN 0001-706X

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Abstract

Leishmania (Mundinia) orientalis is a newly described species causing human leishmaniasis in Thailand whose natural vector is unknown. L. orientalis infections in sand flies and/or biting midges under laboratory conditions have not been previously investigated. In this study, the development of L. orientalis in two experimental vectors, Lutzomyia longipalpis sand flies and Culicoides sonorensis biting midges was investigated for the first time using light microscopy, scanning electron microscopy, and histological examination. The results showed that L. orientalis was unable to establish infection in Lu. longipalpis. No parasites were found in the sand fly gut 4 days post-infected blood meal (PIBM). In contrast, the parasite successfully established infection in C. sonorensis. The parasites differentiated from amastigotes to procyclic promastigotes in the abdominal midgut (AMG) on day 1 PIBM. On day 2 PIBM, nectomonad promastigotes were observed in the AMG and migrated to the thoracic midgut (TMG). Leptomonad promastigotes appeared at the TMG on day 3 PIBM. Clusters of leptomonad promastigotes and metacyclic promastigotes colonized around the stomodeal valve with the accumulation of a promastigote secretory gel-like material from day 3 PIBM onwards. Haptomonad-like promastigotes were observed from day 5 PIBM, and the proportion of metacyclic promastigotes reached 23% on day 7 PIBM. The results suggest that biting midges or other sand fly genera or species might be vectors of L. orientalis.

Item Type:

Journal Article

Journal or Publication Title:

Acta Tropica

Additional Information:

This is the author’s version of a work that was accepted for publication in Acta Tropica. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Acta Tropica, 199, 2019 DOI: 10.1016/j.actatropica.2019.105157

Uncontrolled Keywords:

/dk/atira/pure/subjectarea/asjc/2700/2725

Subjects:

?? leishmania orientalismundinialutzomyia longipalpisculicoides sonorensisleishmaniasisthailandinfectious diseasesparasitology ??

Departments:

Faculty of Health and Medicine > Biomedical & Life Sciences

ID Code:

136727

Deposited By:

ep_importer_pure

Deposited On:

19 Nov 2019 09:30

Refereed?:

Yes

Published?:

Published

Last Modified:

18 Sep 2024 00:53

URI:

https://eprints.lancs.ac.uk/id/eprint/136727