Defining the Identity and Dynamics of Adult Gastric Isthmus Stem Cells

Han, Seungmin and Fink, Juergen and Jörg, David J and Lee, Eunmin and Yum, Min Kyu and Chatzeli, Lemonia and Merker, Sebastian R and Josserand, Manon and Trendafilova, Teodora and Andersson-Rolf, Amanda and Dabrowska, Catherine and Kim, Hyunki and Naumann, Ronald and Lee, Ji-Hyun and Sasaki, Nobuo and Mort, Richard Lester and Basak, Onur and Clevers, Hans and Stange, Daniel E and Philpott, Anna and Kim, Jong Kyoung and Simons, Benjamin D and Koo, Bon-Kyoung (2019) Defining the Identity and Dynamics of Adult Gastric Isthmus Stem Cells. Cell Stem Cell, 25 (3). 342-356.e7. ISSN 1934-5909

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The gastric corpus epithelium is the thickest part of the gastrointestinal tract and is rapidly turned over. Several markers have been proposed for gastric corpus stem cells in both isthmus and base regions. However, the identity of isthmus stem cells (IsthSCs) and the interaction between distinct stem cell populations is still under debate. Here, based on unbiased genetic labeling and biophysical modeling, we show that corpus glands are compartmentalized into two independent zones, with slow-cycling stem cells maintaining the base and actively cycling stem cells maintaining the pit-isthmus-neck region through a process of "punctuated" neutral drift dynamics. Independent lineage tracing based on Stmn1 and Ki67 expression confirmed that rapidly cycling IsthSCs maintain the pit-isthmus-neck region. Finally, single-cell RNA sequencing (RNA-seq) analysis is used to define the molecular identity and lineage relationship of a single, cycling, IsthSC population. These observations define the identity and functional behavior of IsthSCs.

Item Type:
Journal Article
Journal or Publication Title:
Cell Stem Cell
Uncontrolled Keywords:
?? gastric corpus isthmus stem celltwo stem cell compartmentspunctuated neutral driftunbiased genetic labelingdeep tissue imagingbiophysical modelingsingle-cell rna-seqlgr5troyintestinecell biologygeneticsmolecular medicine ??
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Deposited On:
29 Aug 2019 09:10
Last Modified:
15 Jul 2024 19:48