FLCN, a novel autophagy component, interacts with GABARAP and is regulated by ULK1 phosphorylation

Dunlop, Elaine A. and Seifan, Sara and Claessens, Tijs and Behrends, Christian and Kamps, Miriam A.F. and Rozycka, Ewelina and Kemp, Alain J. and Nookala, Ravi K. and Blenis, John and Coull, Barry J. and Murray, James T. and Van Steensel, Maurice A.M. and Wilkinson, Simon and Tee, Andrew R. (2014) FLCN, a novel autophagy component, interacts with GABARAP and is regulated by ULK1 phosphorylation. Autophagy, 10 (10). pp. 1749-1760. ISSN 1554-8627

Full text not available from this repository.

Abstract

Birt-Hogg-Dubé (BHD) syndrome is a rare autosomal dominant condition caused by mutations in the FLCN gene and characterized by benign hair follicle tumors, pneumothorax, and renal cancer. Folliculin (FLCN), the protein product of the FLCN gene, is a poorly characterized tumor suppressor protein, currently linked to multiple cellular pathways. Autophagy maintains cellular homeostasis by removing damaged organelles and macromolecules. Although the autophagy kinase ULK1 drives autophagy, the underlying mechanisms are still being unraveled and few ULK1 substrates have been identified to date. Here, we identify that loss of FLCN moderately impairs basal autophagic flux, while re-expression of FLCN rescues autophagy. We reveal that the FLCN complex is regulated by ULK1 and elucidate 3 novel phosphorylation sites (Ser406, Ser537, and Ser542) within FLCN, which are induced by ULK1 overexpression. In addition, our findings demonstrate that FLCN interacts with a second integral component of the autophagy machinery, GABA(A) receptor-associated protein (GABARAP). The FLCN-GABARAP association is modulated by the presence of either folliculin-interacting protein (FNIP)-1 or FNIP2 and further regulated by ULK1. As observed by elevation of GABARAP, sequestome 1 (SQSTM1) and microtubule-associated protein 1 light chain 3 (MAP1LC3B) in chromophobe and clear cell tumors from a BHD patient, we found that autophagy is impaired in BHD-associated renal tumors. Consequently, this work reveals a novel facet of autophagy regulation by ULK1 and substantially contributes to our understanding of FLCN function by linking it directly to autophagy through GABARAP and ULK1.

Item Type:
Journal Article
Journal or Publication Title:
Autophagy
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1300/1307
Subjects:
ID Code:
136251
Deposited By:
Deposited On:
21 Aug 2019 14:00
Refereed?:
Yes
Published?:
Published
Last Modified:
04 Jul 2020 05:59