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Equine arteritis virus induced cell death is associated with activation of the intrinsic apoptotic signalling pathway

Cholleti, Harindranath and Paidikondala, Maruthibabu and Munir, Muhammad and Hakhverdyan, Mikhayil and Baule, Claudia (2013) Equine arteritis virus induced cell death is associated with activation of the intrinsic apoptotic signalling pathway. Virus Research, 171 (1). pp. 222-226. ISSN 0168-1702

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Abstract

Equine arteritis virus (EAV) causes a respiratory and reproductive disease in horses, equine viral arteritis. Though cell death in infection with EAV is considered to occur by apoptosis, the underlying molecular mechanism has not been extensively elucidated. We investigated the expression of mRNA of pro-apoptotic and caspase genes during EAV infection in BHK21 cells, a well-established cell type for EAV replication. Using a SYBR Green real-time PCR, mRNA of p53, Bax, caspase 3 and caspase 9 were found up-regulated in a time dependent manner in EAV infected cells. Western blot analysis for caspase 3 and caspase 9 showed expression of cleaved forms of these proteins during EAV infection. In addition, a luminescence-based cell assay for caspase 3/7 activation as a hallmark in apoptosis confirmed apoptotic cell death. The findings demonstrate that cell death in EAV infected BHK21 cells results from apoptosis mediated through the intrinsic signalling pathway.

Item Type: Journal Article
Journal or Publication Title: Virus Research
Uncontrolled Keywords: /dk/atira/pure/subjectarea/asjc/1300/1306
Subjects:
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 134791
Deposited By: ep_importer_pure
Deposited On: 22 Jun 2019 09:19
Refereed?: Yes
Published?: Published
Last Modified: 01 Jan 2020 12:04
URI: https://eprints.lancs.ac.uk/id/eprint/134791

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