Establishment of stably transfected cells constitutively expressing the full-length and truncated antigenic proteins of two genetically distinct mink astroviruses

Bidokhti, Mehdi R.M. and Ullman, Karin and Jensen, Trine H. and Chriél, Mariann and Mottahedin, Amin and Munir, Muhammad and Andersson, Anna Maria and Detournay, Olivier and Hammer, Anne Sofie and Baule, Claudia (2013) Establishment of stably transfected cells constitutively expressing the full-length and truncated antigenic proteins of two genetically distinct mink astroviruses. PLoS ONE, 8 (12). ISSN 1932-6203

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Abstract

Astroviruses are becoming a growing concern in veterinary and public health. To date there are no registered vaccines against astrovirus-induced disease, mostly due to the difficulty to cultivate astroviruses to high titer for vaccine development using conventional techniques. As means to circumvent this drawback, we have developed stably transfected mink fetal cells and BHK21 cells constitutively expressing the full-length and truncated capsid proteins of two distinct genotypes of mink astrovirus. Protein expression in these stably transfected cells was demonstrated by strong signals as evaluated by in-situ PLA and IFA, and confirmed by Western blotting. The recombinant full-length and truncated proteins induced a high level of antibodies in mink, evaluated by ELISA, demonstrating their immunogenicity. In a challenge experiment in mink, a reduction in presentation clinical signs and virus shedding was observed in mink kits born from immunized females. The gene integration and protein expression were sustained through cell passage, showing that the used approach is robust and reliable for expression of functional capsid proteins for vaccine and diagnostic applications.

Item Type: Journal Article
Journal or Publication Title: PLoS ONE
Uncontrolled Keywords: /dk/atira/pure/subjectarea/asjc/2700
Subjects:
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 134782
Deposited By: ep_importer_pure
Deposited On: 22 Jun 2019 09:19
Refereed?: Yes
Published?: Published
Last Modified: 15 Jan 2020 05:13
URI: https://eprints.lancs.ac.uk/id/eprint/134782

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