Isolation of oviposition pheromone from the eggs of the sandfly Lutzomyia longipalpis

DOUGHERTY, M. J. and HAMILTON, J. G.C. and Ward, R. D. (1994) Isolation of oviposition pheromone from the eggs of the sandfly Lutzomyia longipalpis. Medical and Veterinary Entomology, 8 (2). pp. 119-124. ISSN 0269-283X

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Abstract

Abstract. Semiochemical components of eggs of the sandfly Lutzomyia longipalpis (Diptera: Psychodidae) were separated by high performance liquid chromatography. HPLC fractions were examined quantitatively and qualitatively by gas chromatography (GC). A bioassay was used to determine the oviposition attraction of gravid L. longipalpis to each of the fractions separately and a peak responsible for the semiochemical activity was identified. Gravid flies were placed in individual oviposition tubes to determine if the peak of interest was an oviposition stimulant. The active semiochemical fraction attracted gravid flies for oviposition. Furthermore, egg laying was enhanced: gravid flies exposed to the pheromone oviposited earlier and laid more eggs than control flies. GC analysis indicated that 1200 eggs (2 days old) gave a yield of 12.75 ug of active pheromone. This fraction had similar HPLC and GC retention times to caryophyllene oxide, suggesting comparable polarity and molecular weight.

Item Type:
Journal Article
Journal or Publication Title:
Medical and Veterinary Entomology
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1100/1109
Subjects:
ID Code:
134453
Deposited By:
Deposited On:
22 Jun 2019 08:53
Refereed?:
Yes
Published?:
Published
Last Modified:
03 Mar 2020 04:48