Developing A Synthetic Composite Membrane For Cleft Palate Repair

Sharif, Faiza and Roman, Sabiniano and Asif, Anila and Gigliobianco, Giulia and Ghafoor, Sarah and Tariq, Muhammad and Siddiqui, Saadat Anwer and Mahmood, Farrukh and Muhammad, Nawshad and Rehman, Ihtesham Ur and MacNeil, Sheila (2019) Developing A Synthetic Composite Membrane For Cleft Palate Repair. Journal of Tissue Engineering and Regenerative Medicine, 13 (7). pp. 1178-1189. ISSN 1932-6254

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Abstract

An oronasal fistula is a passage between the oral and nasal cavity. Currently, surgical procedures use mucosal flaps or collagen grafts to make a barrier between oral and nasal cavities. Our aim was to develop a cell-free synthetic repair material for closure of nasal fistulas. We surface functionalized electrospun polyurethane (PU) and poly-L-lactic acid (PLLA) and composite polymer (PU-PLLA) membranes with acrylic acid through plasma polymerization. Membranes were treated in a layer-by-layer approach to develop highly charged electrostatic layer that could bind heparin as a pro-angiogenic glycosaminoglycan. The properties were evaluated through physical, chemical, and mechanical characterization techniques. Cytotoxicity was tested with MC3T3 pre-osteoblast cell lines for 3, 7, and 14 days, and vasculogenesis was assessed by implantation into the chorio-allantoic membrane in chick embryos for 7 days. In vivo biocompatibility was assessed by subcutaneous implantation in rats for 1, 3, and 6 weeks. The membranes consisted of random fibers of PLLA-PU with fiber diameters of 0.47 and 0.12 μm, respectively. Significantly higher cell proliferation and migration of MC3T3 cells at 3, 7, and 14 days were shown on plasma-coated membranes compared with uncoated membranes. Further, it was found that plasma-coated membranes were more angiogenic than controls. In vivo implantation of membranes in rats did not reveal any gross toxicity to the materials, and wound healing was comparable with the native tissue repair (sham group). We therefore present a plasma-functionalized electrospun composite polymer membrane for use in the treatment of fistulas. These membranes are flexible, non-cytotoxic, and angiogenic, and we hope it should lead to permanent closure of oronasal fistula.

Item Type:
Journal Article
Journal or Publication Title:
Journal of Tissue Engineering and Regenerative Medicine
Additional Information:
This is the peer reviewed version of the following article: Sharif, F, Roman, S, Asif, A, et al. Developing a synthetic composite membrane for cleft palate repair. J Tissue Eng Regen Med. 2019; 13(7), 1178-1189. https://doi.org/10.1002/term.2867 which has been published in final form at https://onlinelibrary.wiley.com/doi/full/10.1002/term.2867 This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/2700/2701
Subjects:
ID Code:
133658
Deposited By:
Deposited On:
07 May 2019 14:20
Refereed?:
Yes
Published?:
Published
Last Modified:
24 Oct 2020 06:27