Functionalization of thioctic acid-capped gold nanoparticles for specific immobilization of histidine-tagged proteins

Abad, J.M. and Mertens, S.F.L. and Pita, M. and Fernández, V.M. and Schiffrin, D.J. (2005) Functionalization of thioctic acid-capped gold nanoparticles for specific immobilization of histidine-tagged proteins. Journal of the American Chemical Society, 127 (15). pp. 5689-5694. ISSN 0002-7863

Full text not available from this repository.

Abstract

This paper presents an efficient strategy for the specific immobilization of fully functional proteins onto the surface of nanoparticles. Thioctic acid-derivatized gold clusters are used as a scaffold for further stepwise modification, leading to a cobalt(II)-terminated ligand shell. A histidine tag introduced by genetic engineering into a protein is coordinated to this transition metal ion. The specific immobilization has been demonstrated for the cases of a genetically engineered horseradish peroxidase and ferredoxin-NADP+ reductase, confirming the attachment of the fully functional proteins to the Co(II)-terminated nanointerface. The absence of nonspecific protein adsorption and the specificity of the binding site have been verified using several analogues of the enzymes without the histidine tag. © 2005 American Chemical Society.

Item Type: Journal Article
Journal or Publication Title: Journal of the American Chemical Society
Uncontrolled Keywords: /dk/atira/pure/subjectarea/asjc/1500/1503
Subjects:
ID Code: 133002
Deposited By: ep_importer_pure
Deposited On: 22 Apr 2019 11:05
Refereed?: Yes
Published?: Published
Last Modified: 19 Feb 2020 05:21
URI: https://eprints.lancs.ac.uk/id/eprint/133002

Actions (login required)

View Item View Item