Mesenchymal stem cell (MSC) viability on PVA and PCL polymer coated hydroxyapatite scaffolds derived from cuttlefish

Siddiqi, S.A. and Manzoor, F. and Jamal, A. and Tariq, M. and Ahmad, R. and Kamran, M. and Chaudhry, A. and Rehman, I.U. (2016) Mesenchymal stem cell (MSC) viability on PVA and PCL polymer coated hydroxyapatite scaffolds derived from cuttlefish. RSC Advances, 6 (39). pp. 32897-32904. ISSN 2046-2069

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Abstract

In the present study, cuttlefish bones are used to prepare highly porous hydroxyapatite (HA) scaffolds via hydrothermal treatment at 200 °C. Raw cuttlefish bones (CB) and the hydrothermal products have been analyzed and compared for their composition and microstructure, using X-ray powder diffraction (XRD), Optical Microscopy (OM), Scanning Electron Microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), porosity estimation and compressive strength measuring techniques. Characterization reveals that cuttlebone has high porosity approaching above 70%, and possesses the laminar structure of aragonite mixed with some organic materials. The compressive strength of the CB-HA is improved after coating with both polyvinyl alcohol (PVA) and polycaprolactone (PCL). Furthermore, our in vitro biocompatibility studies revealed that CB-HA and PVA coated CB-HA scaffolds are non-cytotoxic and support the adherence and proliferation of rMSCs, comparable to pure HA scaffolds. Altogether, our results suggest that naturally derived CB-HA, PVA and PCL coated CB-HA scaffolds are potential cheap candidates for bone tissue engineering applications, and also that PVA and PCL coatings provide better mechanical strength. © The Royal Society of Chemistry 2016.

Item Type:
Journal Article
Journal or Publication Title:
RSC Advances
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1600
Subjects:
ID Code:
132824
Deposited By:
Deposited On:
16 Apr 2019 09:20
Refereed?:
Yes
Published?:
Published
Last Modified:
23 Jan 2020 05:12