Raman spectroscopy detects melanoma and the tissue surrounding melanoma using tissue-engineered melanoma models

Yorucu, C. and Lau, K. and Mittar, S. and Green, N.H. and Raza, A. and Rehman, I.U. and MacNeil, S. (2016) Raman spectroscopy detects melanoma and the tissue surrounding melanoma using tissue-engineered melanoma models. APPLIED SPECTROSCOPY REVIEWS, 51 (4). pp. 263-277. ISSN 0570-4928

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Abstract

Invasion of melanoma cells from the primary tumor involves interaction with adjacent tissues and extracellular matrix. The extent of this interaction is not fully understood. In this study Raman spectroscopy was applied to cryo-sections of established 3D models of melanoma in human skin. Principal component analysis was used to investigate differences between the tumor and normal tissue and between the peri-tumor area and the normal skin. Two human melanoma cells lines A375SM and C8161 were investigated and compared in 3D melanoma models. Changes were found in protein conformations and tryptophan configurations across the entire melanoma samples, in tyrosine orientation and in more fluid lipid packing only in tumor dense areas, and in increased glycogen content in the peri-tumor areas of melanoma. Raman spectroscopy revealed changes around the perimeter of a melanoma tumor as well as detecting differences between the tumor and the normal tissue. © 2016 Ceyla Yorucu, Katherine Lau, Shweta Mittar, Nicola H. Green, Ahtasham Raza, Ihtesham Ur Rehman, and Sheila MacNeil.

Item Type:
Journal Article
Journal or Publication Title:
APPLIED SPECTROSCOPY REVIEWS
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1600/1607
Subjects:
ID Code:
132822
Deposited By:
Deposited On:
16 Apr 2019 09:30
Refereed?:
Yes
Published?:
Published
Last Modified:
17 Jul 2020 12:55