Triethyl orthoformate covalently cross-linked chitosan-(poly vinyl) alcohol based biodegradable scaffolds with heparin-binding ability for promoting neovascularisation

Shahzadi, L. and Yar, M. and Jamal, A. and Siddiqi, S.A. and Chaudhry, A.A. and Zahid, S. and Tariq, M. and Rehman, I.U. and MacNeil, S. (2016) Triethyl orthoformate covalently cross-linked chitosan-(poly vinyl) alcohol based biodegradable scaffolds with heparin-binding ability for promoting neovascularisation. JOURNAL OF BIOMATERIALS APPLICATIONS, 31 (4). pp. 582-593. ISSN 0885-3282

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Abstract

There is a need to develop pro-angiogenic biomaterials to promote wound healing and to assist in regenerative medicine. To this end, various growth factors have been exploited which have the potential to promote angiogenesis. However, these are generally expensive and labile which limits their effectiveness. An alternative approach is to immobilize heparin onto biocompatible degradable hydrogels. The heparin in turn will then bind endogenous proangiogenic growth factors to induce formation of new blood vessels. In this study, we continue our development of hydrogels for wound healing purposes by exploring covalently cross-linking chitosan and polyvinyl alcohol hydrogels using triethyl orthoformate. Two concentrations of triethyl orthoformate (4 and 16%) were compared for their effects on the structure of hydrogels - their swelling, pore size, and rate of degradation and for their ability to support the growth of cells and for their heparin-binding capacity and their effects on angiogenesis in a chick chorioallantoic membrane assay. Hydrogels formed with 4 or 16% both triethyl orthoformate cross-linker were equally cyto-compatible. Hydrogels formed with 4% triethyl orthoformate absorbed slightly more water than those made with 16% triethyl orthoformate and broke down slightly faster than non-cross-linked hydrogels. When soaked in heparin the hydrogel formed with 16% triethyl orthoformate showed more blood vessel formation in the CAM assay than that formed with 4% triethyl orthoformate. © The Author(s) 2016.

Item Type:
Journal Article
Journal or Publication Title:
JOURNAL OF BIOMATERIALS APPLICATIONS
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/2200/2204
Subjects:
?? angiogenesischitosancross-linked chitosanheparinhydrogelsbinsbiocompatibilityblood vesselschitincrosslinkingpolysaccharidespore sizeangiogenesisbiodegradable scaffoldchick chorioallantoic membranecovalently cross-linkedcross linked chitosancross-linked hy ??
ID Code:
132816
Deposited By:
Deposited On:
16 Apr 2019 09:35
Refereed?:
Yes
Published?:
Published
Last Modified:
15 Jul 2024 19:16