Chitosan hydrogels enriched with polyphenols:Antibacterial activity, cell adhesion and growth and mineralization

Liskova, Jana and Douglas, Timothy E. L. and Beranova, Jana and Skwarczynska, Agata and Bozic, Mojca and Samal, Sangram Keshari and Modrzejewska, Zofia and Gorgieva, Selestina and Kokol, Vanja and Bacakova, Lucie (2015) Chitosan hydrogels enriched with polyphenols:Antibacterial activity, cell adhesion and growth and mineralization. Carbohydrate Polymers, 129. pp. 135-142. ISSN 0144-8617

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Abstract

Injectable hydrogels for bone regeneration consisting of chitosan, sodium beta-glycerophosphate (Na-β-GP) and alkaline phosphatase (ALP) were enriched with the polyphenols phloroglucinol (PG) and gallic acid (GA) and characterized physicochemically and biologically with respect to properties relevant for applications in bone regeneration, namely gelation kinetics, mineralizability, antioxidant properties, antibacterial activity, cytocompatibility and ability to support adhesion and growth of human osteoblast-like MG63 cells. Enrichment with PG and GA had no negative effect on gelation kinetics and mineralizability. PG and GA both enhanced antioxidant activity of unmineralized hydrogels. Mineralization reduced antioxidant activity of hydrogels containing GA. Hydrogels containing GA, PG and without polyphenols reduced colony forming ability of Escherichia coli after 1 h, 3 h and 6 h incubation and slowed E. coli growth in liquid culture for 150 min. Hydrogels containing GA were cytotoxic and supported cell growth more poorly than polyphenol-free hydrogels. PG had no negative effect on cell adhesion and growth.

Item Type:
Journal Article
Journal or Publication Title:
Carbohydrate Polymers
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/2500/2507
Subjects:
ID Code:
132328
Deposited By:
Deposited On:
02 Apr 2019 09:30
Refereed?:
Yes
Published?:
Published
Last Modified:
11 Sep 2020 04:52