Pulsed laser deposition of magnesium-doped calcium phosphate coatings on porous polycaprolactone scaffolds produced by rapid prototyping

Vandrovcova, Marta and Douglas, Timothy E.L. and Mróz, Waldemar and Musial, Olga and Schaubroeck, David and Budner, Boguslaw and Syroka, Renata and Dubruel, Peter and Bacakova, Lucie (2015) Pulsed laser deposition of magnesium-doped calcium phosphate coatings on porous polycaprolactone scaffolds produced by rapid prototyping. Materials Letters, 148. pp. 178-183. ISSN 0167-577X

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Abstract

Polycaprolactone (PCL) is a biodegradable and biocompatible polyester whose low melting point facilitates production of 3D porous scaffolds with precisely defined dimensions and internal architecture by rapid prototyping techniques. To improve the suitability of such PCL scaffolds for bone regeneration applications, they were coated with inorganic layers of calcium phosphate (CaP) and CaP doped with 0.6% w/v magnesium (CaP+Mg) using pulsed laser deposition (PLD) and characterized in vitro using osteoblast-like Saos-2 cells. Saos-2 cells were able to adhere to all scaffolds. CaP+Mg coatings significantly increased activity of alkaline phosphatase (ALP), an early differentiation marker, after 7 days. However, gene expression of ALP after 7 days was markedly lower on the same scaffolds. These data show the feasibility of coating PCL with CaP layers by PLD and the possibility of influencing osteoblastic differentiation by magnesium doping of the CaP coating.

Item Type:
Journal Article
Journal or Publication Title:
Materials Letters
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/3100/3104
Subjects:
ID Code:
132284
Deposited By:
Deposited On:
01 Apr 2019 16:20
Refereed?:
Yes
Published?:
Published
Last Modified:
11 Jun 2020 07:49