Enzymatically biomineralized chitosan scaffolds for tissue-engineering applications

Dash, Mamoni and Samal, Sangram K. and Douglas, Timothy E. L. and Schaubroeck, David and Leeuwenburgh, Sander C. and Voort, Pascal Van Der and Declercq, Heidi A. and Dubruel, Peter (2017) Enzymatically biomineralized chitosan scaffolds for tissue-engineering applications. Journal of Tissue Engineering and Regenerative Medicine, 11 (5). pp. 1500-1513. ISSN 1932-6254

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Abstract

Porous biodegradable scaffolds represent promising candidates for tissue‐engineering applications because of their capability to be preseeded with cells. We report an uncrosslinked chitosan scaffold designed with the aim of inducing and supporting enzyme‐mediated formation of apatite minerals in the absence of osteogenic growth factors. To realize this, natural enzyme alkaline phosphatase (ALP) was incorporated into uncrosslinked chitosan scaffolds. The uncrosslinked chitosan makes available amine and alcohol functionalities to enhance the biomineralization process. The physicochemical findings revealed homogeneous mineralization, with the phase structure of the formed minerals resembling that of apatite at low mineral concentrations, and similar to dicalcium phosphate dihydrate (DCPD) with increasing ALP content. The MC3T3 cell activity clearly showed that the mineralization of the chitosan scaffolds was effective in improving cellular adhesion, proliferation and colonization.

Item Type:
Journal Article
Journal or Publication Title:
Journal of Tissue Engineering and Regenerative Medicine
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/2700/2701
Subjects:
ID Code:
132283
Deposited By:
Deposited On:
02 Apr 2019 09:25
Refereed?:
Yes
Published?:
Published
Last Modified:
01 Apr 2020 07:14