An analysis of benign human prostate offers insight into the mechanism of apocrine secretion and the origin of prostasomes

Fullwood, Nigel James and Lawlor, Alan J. and Martin-Hirsch, Pierre Leonard and Matanhelia, Shyam and Martin, Francis (2019) An analysis of benign human prostate offers insight into the mechanism of apocrine secretion and the origin of prostasomes. Scientific Reports, 9. ISSN 2045-2322

Full text not available from this repository.

Abstract

The structure and function of normal human prostate is still not fully understood. Herein, we concentrate on the different cell types present in normal prostate, describing some previously unreported types and provide evidence that prostasomes are primarily produced by apocrine secretion. Patients (n = 10) undergoing TURP were prospectively consented based on their having a low risk of harbouring CaP. Scanning electron microscopy and transmission electron microscopy was used to characterise cell types and modes of secretion. Zinc levels were determined using Inductively Coupled Plasma Mass Spectrometry. Although merocrine secretory cells were noted, the majority of secretory cells appear to be apocrine; for the first time, we clearly show high-resolution images of the stages of aposome secretion in human prostate. We also report a previously undescribed type of epithelial cell and the first ultrastructural image of wrapping cells in human prostate stroma. The zinc levels in the tissues examined were uniformly high and X-ray microanalysis detected zinc in merocrine cells but not in prostasomes. We conclude that a significant proportion of prostasomes, possibly the majority, are generated via apocrine secretion. This finding provides an explanation as to why so many large proteins, without a signal peptide sequence, are present in the prostatic fluid.

Item Type: Journal Article
Journal or Publication Title: Scientific Reports
Uncontrolled Keywords: /dk/atira/pure/subjectarea/asjc/1000
Subjects:
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 131611
Deposited By: ep_importer_pure
Deposited On: 25 Feb 2019 10:20
Refereed?: Yes
Published?: Published
Last Modified: 14 Sep 2019 03:08
URI: https://eprints.lancs.ac.uk/id/eprint/131611

Actions (login required)

View Item View Item