Genotoxicity and cytotoxicity of the plasma jet-treated medium on lymphoblastoid WIL2-NS cell line using the cytokinesis block micronucleus cytome assay

Hong, Sung-Ha and Szili, Endre J. and Fenech, Michael and Gaur, Nishtha and Short, Robert D (2017) Genotoxicity and cytotoxicity of the plasma jet-treated medium on lymphoblastoid WIL2-NS cell line using the cytokinesis block micronucleus cytome assay. Scientific Reports. ISSN 2045-2322

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Abstract

Despite growing interest in the application of atmospheric plasma jets as medical treatment strategies, there has been comparatively little research on the potential genotoxic and cytotoxic effects of plasma jet treatment. In this study, we have employed the cytokinesis block micronucleus cytome (CBMN-Cyt) assay with WIL2-NS B lymphoblastoid cells to test the potential genotoxicity, as well as the cytotoxicity, of toxic species generated in cell culture media by an argon (Ar) plasma jet. Elevated levels of cell death (necrosis) and occurrence of chromosomal damage (micronuclei MN, nculeoplasmic bridge NPBs and nuclear bus, Nbuds) were observed when cells were exposed to plasma jet-treated media. These results provide a first insight into how we might measure the genotoxic and cytotoxic effect of plasma jet treatments (both indirect and direct) in dividing human cells.

Item Type: Journal Article
Journal or Publication Title: Scientific Reports
Uncontrolled Keywords: /dk/atira/pure/subjectarea/asjc/1000
Subjects:
ID Code: 131002
Deposited By: ep_importer_pure
Deposited On: 01 Feb 2019 15:50
Refereed?: Yes
Published?: Published
Last Modified: 19 Feb 2020 05:11
URI: https://eprints.lancs.ac.uk/id/eprint/131002

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