Limiting Pseudomonas aeruginosa Biofilm Formation Using Cold Atmospheric Pressure Plasma

Patenall, Bethany L and Hathaway, Hollie and Sedgwick, Adam C. and Thet, Naing T. and Williams, George T. and Young, Amber E. and Allinson, Sarah Louise and Short, Robert D and Jenkins, A. Toby A. (2018) Limiting Pseudomonas aeruginosa Biofilm Formation Using Cold Atmospheric Pressure Plasma. Plasma Medicine, 8 (3). pp. 269-277. ISSN 1947-5764

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Abstract

We investigate the ability to disrupt and limit growth biofilms of Pseudomonas aeruginosa using application of cold atmospheric pressure (CAP) plasma. The effect of the bio-film's exposure to a helium (CAP) jet was assessed at varying time points during biofilm maturation. Results showed that the amount of time during biofilm growth that CAP pressure was applied has a crucial role on the ability of biofilms to mature and recover after CAP exposure. Intervention during the early stages of biofilm formation (0-8 h) results in a 4-5-log reduction in viable bacterial cells (measured at 24 h of incubation) relative to untreated biofilms. However, CAP treatment of biofilm at 12 h and above only results in a 2-log reduction in viable cells. This has potentially important implications for future clinical application of CAP to treat infected wounds.