Enhancing resolution and sensitivity of 17O solid-state NMR through combining double rotation, 1H decoupling and satellite modulation for biomolecular applications

Howes, A.P. and Anupõld, T. and Lemaitre, V. and Kukol, A. and Watts, A. and Samoson, A. and Smith, M.E. and Dupree, R. (2006) Enhancing resolution and sensitivity of 17O solid-state NMR through combining double rotation, 1H decoupling and satellite modulation for biomolecular applications. Chemical Physics Letters, 421 (1-3). pp. 42-46. ISSN 0009-2614

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Abstract

17O solid-state NMR is a highly sensitive probe of structural detail of organic solids but improvements in sensitivity and resolution are crucial for it to be applied to larger biological molecules. Here it is shown that high resolution (∼1 ppm) and significant signal enhancement can be achieved by combining 1H decoupled double rotation (DOR), which narrows the lines by a factor of ∼100 compared to conventional magic angle spinning, and manipulation of the satellite transition populations to transfer magnetisation to the central transition, which produces a signal enhancement of ∼2. © 2006 Elsevier B.V. All rights reserved.

Item Type:
Journal Article
Journal or Publication Title:
Chemical Physics Letters
Subjects:
?? centralized signal controlmagnetizationmodulationmolecular orientationsensitivity analysisangle spinningbiomolecular applicationsdecoupled double rotation (dor)satellite transition populationsnuclear magnetic resonance ??
Departments:
ID Code:
130321
Deposited By:
Deposited On:
04 Jan 2019 11:40
Refereed?:
Yes
Published?:
Published
Last Modified:
15 Jul 2024 18:46