Douglas, Timothy Edward Lim and Dziadek, Michal and Schietse, Josefien and Boone, Matthieu and Declercq, Heidi and Coenye, Tom and Vanhoorne, Valerie and Vervaet, Chris and Balcaen, Lieve and Buchweitz, Maria and Vanhaecke, Frank and Van Assche, Frederic and Cholewa-Kowalska, Katarzyna and Skirtach, Andre G. (2019) Pectin-bioactive glass self-gelling, injectable composites with high antibacterial activity. Carbohydrate Polymers, 205. pp. 427-436. ISSN 0144-8617
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Abstract
The present work focuses on the development of novel injectable, self-gelling composite hydrogels based on two types of low esterified amidated pectins from citrus peels and apple pomace. Sol-gelderived, calcium-rich bioactive glass (BG) fillers in a particle form are applied as delivery vehicles for the release of Ca2+ ions to induce internal gelation of pectins. Composites were prepared by a relatively simple mixing technique, using 20% w/v BG particles of two different sizes (2.5 and <45 µm). Smaller particles accelerated pectin gelation slightly faster than bigger ones, which appears to result from the higher rate of Ca2+ ion release. µCT showed inhomogeneous distribution of the BG particles within the hydrogels. All composite hydrogels exhibited strong antibacterial activity against methicilin-resistant Staphylococcus aureus. The mineralization process of pectin-BG composite hydrogels occurred upon incubation in simulated body fluid for 28 days. In vitro studies demonstrated cytocompatibility of composite hydrogels with MC3T3-E1 osteoblastic cells.