Cell density and airspace patterning in the leaf can be manipulated to increase leaf photosynthetic capacity

Lehmeier, Christoph and Pajor, Radoslaw and Lundgren, Marjorie R. and Mathers, Andrew and Sloan, Jen and Bauch, Marion and Mitchell, Alice and Bellasio, Chandra and Green, Adam and Bouyer, Daniel and Schnittger, Arp and Sturrock, Craig and Osborne, Colin P. and Rolfe, Stephen and Mooney, Sacha and Fleming, Andrew J. (2017) Cell density and airspace patterning in the leaf can be manipulated to increase leaf photosynthetic capacity. Plant Journal, 92 (6). pp. 981-994. ISSN 0960-7412

Abstract

The pattern of cell division, growth and separation during leaf development determines the pattern and volume of airspace in a leaf. The resulting balance of cellular material and airspace is expected to significantly influence the primary function of the leaf, photosynthesis, and yet the manner and degree to which cell division patterns affect airspace networks and photosynthesis remains largely unexplored. In this paper we investigate the relationship of cell size and patterning, airspace and photosynthesis by promoting and repressing the expression of cell cycle genes in the leaf mesophyll. Using microCT imaging to quantify leaf cellular architecture and fluorescence/gas exchange analysis to measure leaf function, we show that increased cell density in the mesophyll of Arabidopsis can be used to increase leaf photosynthetic capacity. Our analysis suggests that this occurs both by increasing tissue density (decreasing the relative volume of airspace) and by altering the pattern of airspace distribution within the leaf. Our results indicate that cell division patterns influence the photosynthetic performance of a leaf, and that it is possible to engineer improved photosynthesis via this approach.