Spontaneous charged lipid transfer between lipid vesicles

Richens, Joanna L. and Tyler, Arwen I.I. and Barriga, Hanna M.G. and Bramble, Jonathan P. and Law, Robert V. and Brooks, Nicholas J. and Seddon, John M. and Ces, Oscar and O'Shea, Paul (2017) Spontaneous charged lipid transfer between lipid vesicles. Scientific Reports, 7. ISSN 2045-2322

Full text not available from this repository.

Abstract

© 2017 The Author(s). An assay to study the spontaneous charged lipid transfer between lipid vesicles is described. A donor/acceptor vesicle system is employed, where neutrally charged acceptor vesicles are fluorescently labelled with the electrostatic membrane probe Fluoresceinphosphatidylethanolamine (FPE). Upon addition of charged donor vesicles, transfer of negatively charged lipid occurs, resulting in a fluorescently detectable change in the membrane potential of the acceptor vesicles. Using this approach we have studied the transfer properties of a range of lipids, varying both the h eadgroup and the chain length. At the low vesicle concentrations chosen, the transfer follows a first-order process where lipid monomers are transferred presumably through the aqueous solution phase from donor to acceptor vesicle. The rate of transfer decreases with increasing chain length which is consistent with energy models previously reported for lipid monomer vesicle interactions. Our assay improves on existing methods allowing the study of a range of unmodified lipids, continuous monitoring of transfer and simplified experimental procedures.

Item Type:
Journal Article
Journal or Publication Title:
Scientific Reports
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1000
Subjects:
ID Code:
126034
Deposited By:
Deposited On:
22 Jun 2018 11:00
Refereed?:
Yes
Published?:
Published
Last Modified:
16 Nov 2020 08:14