Evidence for sodium metasilicate receptors on the human osteoblast cell surface; spatial localization and binding properties

Vere, Kelly-Ann and Richens, Joanna L. and Lane, Jordan S. and Harris, Helen J. and Duggan, James and O'Shea, Paul (2013) Evidence for sodium metasilicate receptors on the human osteoblast cell surface; spatial localization and binding properties. Molecular Membrane Biology, 30 (8). pp. 386-393. ISSN 0968-7688

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Abstract

We report details of the interaction of sodium metasilicate with osteoblast cellular membranes using Fluoresceinphosphatidylethanolamine (FPE) as a fluorescent indicator of membrane interactions. Fluorescence imaging studies of the FPE-based indicator system revealed areas of localized binding that would be consistent with the presence of a structure with 'receptor-like' properties. From these results, it seems unlikely that silica binds 'non-specifically' to the osteoblast surface. Moreover, the receptors are localized into membrane domains. Such regions of the cell membrane could well be structures such as 'rafts' or other such localized domains within the membrane. The binding profile of silica with the osteoblast cell surface takes place with all the characteristics of a receptor-mediated process best represented by a cooperativity (sigmoidal) binding model with a Hill coefficient of 3.6.

Item Type:
Journal Article
Journal or Publication Title:
Molecular Membrane Biology
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1300/1312
Subjects:
ID Code:
126031
Deposited By:
Deposited On:
22 Jun 2018 10:38
Refereed?:
Yes
Published?:
Published
Last Modified:
01 Jan 2020 11:24