Analysis of Leishmania mimetic neoglycoproteins for the cutaneous leishmaniasis diagnosis

de Souza, Lígia Moraes Barizon and Thomaz Soccol, Vanete and Petterle, Ricardo Rasmussen and Bates, Michelle D and Bates, Paul A (2018) Analysis of Leishmania mimetic neoglycoproteins for the cutaneous leishmaniasis diagnosis. Parasitology. ISSN 0031-1820

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Abstract

Oligosaccharides are broadly present on Leishmania cell surfaces. They can be useful for the leishmaniases diagnosis and also helpful in identifying new cell markers for the disease. The disaccharide Galα1-3Galβ is the immunodominant saccharide in Leishmania cell surface and is the unique non-reducing terminal glycosphingolipids structure recognized by anti-α-Gal. This study describes an enzyme-linked immunosorbent assay (ELISA) used to measure serum levels of anti-α-galactosyl (α-Gal) antibodies in patients with cutaneous leishmaniasis (CL). Optimal ELISA conditions were established and two neoglycoproteins (NGP) containing the Galα1-3Gal terminal fraction (Galα1-3Galβ1-4GlcNAc-HAS and Galα1-3Gal-HAS) and one Galα1-3Gal NGP analogue (Galα1-3Galβ1-3GlcNAc-HAS) were used as antigens. Means of anti-α-Gal antibody titres of CL patients were significantly higher (P < 0.05) than the healthy individuals for all NGPs tested. Sensitivity and specificity of all NGPs ranged from 62.2 to 78.4% and 58.3 to 96.7%, respectively. In conclusion, the NGPs can be used for CL diagnosis.

Item Type:
Journal Article
Journal or Publication Title:
Parasitology
Additional Information:
https://www.cambridge.org/core/journals/parasitology/article/analysis-of-leishmania-mimetic-neoglycoproteins-for-the-cutaneous-leishmaniasis-diagnosis/101C72EB3721CE2245D73A580D5C17E6 The final, definitive version of this article has been published in the Journal, Parasitology, ? (?), pp ???-??? 2018, © 2018 Cambridge University Press.
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/2400/2405
Subjects:
ID Code:
125812
Deposited By:
Deposited On:
28 Jun 2018 08:10
Refereed?:
Yes
Published?:
Published
Last Modified:
19 Sep 2020 05:15