LFASS: Label-Free Automated Survival Scoring for High-Throughput Nematode Assays

Benedetto, Alex and Bambade, Timothee and Au, Catherine and Tullet, Jennifer and Dang, Hairuo and Cetnar, Kalina and Cabreiro, Filipe and Gems, David (2017) LFASS: Label-Free Automated Survival Scoring for High-Throughput Nematode Assays. Biorxiv.

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Abstract

Caenorhabditis elegans is an excellent model for high-throughput experimental approaches, but lacks a robust, versatile and automated means to pinpoint time of death. Here we describe an automated, label-free, high-throughput method using death-associated fluorescence to monitor nematode survival, which we apply to stress and infection resistance assays. We demonstrate its use to define correlations between age, longevity and stress-resistance, and reveal an autophagy-dependent increase in acute stress resistance in early adulthood.

Item Type: Journal Article
Journal or Publication Title: Biorxiv
Uncontrolled Keywords: /dk/atira/pure/subjectarea/aacsb/disciplinebasedresearch
Subjects:
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 125767
Deposited By: ep_importer_pure
Deposited On: 07 Jun 2018 09:20
Refereed?: No
Published?: Published
Last Modified: 03 Jan 2020 03:13
URI: https://eprints.lancs.ac.uk/id/eprint/125767

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