LFASS: Label-Free Automated Survival Scoring for High-Throughput Nematode Assays

Benedetto, Alex and Bambade, Timothee and Au, Catherine and Tullet, Jennifer and Dang, Hairuo and Cetnar, Kalina and Cabreiro, Filipe and Gems, David (2017) LFASS: Label-Free Automated Survival Scoring for High-Throughput Nematode Assays. Biorxiv.

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Abstract

Caenorhabditis elegans is an excellent model for high-throughput experimental approaches, but lacks a robust, versatile and automated means to pinpoint time of death. Here we describe an automated, label-free, high-throughput method using death-associated fluorescence to monitor nematode survival, which we apply to stress and infection resistance assays. We demonstrate its use to define correlations between age, longevity and stress-resistance, and reveal an autophagy-dependent increase in acute stress resistance in early adulthood.

Item Type:
Journal Article
Journal or Publication Title:
Biorxiv
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/aacsb/disciplinebasedresearch
Subjects:
ID Code:
125767
Deposited By:
Deposited On:
07 Jun 2018 09:20
Refereed?:
No
Published?:
Published
Last Modified:
03 Jul 2020 03:44