Generation of composites for bone tissue-engineering applications consisting of gellan gum hydrogels mineralized with calcium and magnesium phosphate phases by enzymatic means

Douglas, Timothy Edward Lim and Krawczyk, Grzegorz and Pamula, Elzbieta and Declercq, Heidi and Schaubroeck, David and Bucko, Miroslaw and Balcaen, Lieve and Van der Voort, Pascal and Bliznuk, Vitaliy and van den Vreken, Natasja and Dash, Mamoni and Detsch, Rainer and Boccaccini, Aldo and Vanhaecke, Frank and Cornelissen, Ria and Dubruel, (2016) Generation of composites for bone tissue-engineering applications consisting of gellan gum hydrogels mineralized with calcium and magnesium phosphate phases by enzymatic means. Journal of Tissue Engineering and Regenerative Medicine, 10 (11). pp. 938-954. ISSN 1932-6254

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Abstract

Mineralization of hydrogels, desirable for bone regeneration applications, may be achieved enzymatically by incorporation of alkaline phosphatase (ALP). ALP‐loaded gellan gum (GG) hydrogels were mineralized by incubation in mineralization media containing calcium and/or magnesium glycerophosphate (CaGP, MgGP). Mineralization media with CaGP:MgGP concentrations 0.1:0, 0.075:0.025, 0.05:0.05, 0.025:0.075 and 0:0.1 (all values mol/dm3, denoted A, B, C, D and E, respectively) were compared. Mineral formation was confirmed by IR and Raman, SEM, ICP‐OES, XRD, TEM, SAED, TGA and increases in the the mass fraction of the hydrogel not consisting of water. Ca was incorporated into mineral to a greater extent than Mg in samples mineralized in media A–D. Mg content and amorphicity of mineral formed increased in the order A < B < C < D. Mineral formed in media A and B was calcium‐deficient hydroxyapatite (CDHA). Mineral formed in medium C was a combination of CDHA and an amorphous phase. Mineral formed in medium D was an amorphous phase. Mineral formed in medium E was a combination of crystalline and amorphous MgP. Young's moduli and storage moduli decreased in dependence of mineralization medium in the order A > B > C > D, but were significantly higher for samples mineralized in medium E. The attachment and vitality of osteoblastic MC3T3‐E1 cells were higher on samples mineralized in media B–E (containing Mg) than in those mineralized in medium A (not containing Mg). All samples underwent degradation and supported the adhesion of RAW 264.7 monocytic cells, and samples mineralized in media A and B supported osteoclast‐like cell formation.

Item Type: Journal Article
Journal or Publication Title: Journal of Tissue Engineering and Regenerative Medicine
Uncontrolled Keywords: /dk/atira/pure/subjectarea/asjc/2700/2701
Subjects:
Departments: Faculty of Science and Technology > Engineering
ID Code: 125413
Deposited By: ep_importer_pure
Deposited On: 23 May 2018 10:36
Refereed?: Yes
Published?: Published
Last Modified: 19 Feb 2020 04:36
URI: https://eprints.lancs.ac.uk/id/eprint/125413

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