Pectin coatings on titanium alloy scaffolds produced by additive manufacturing:Promotion of human bone marrow stromal cell proliferation

Douglas, Timothy Edward Lim and Hempel, Ute and Zydek, Jagoda and Vladescu, Alina and Pietryga, Krzysztof and Kaeswurm, Julia and Buchweitz, Maria and Surmenev, Roman and Surmeneva, Maria and Cotrut, Cosmin and Koptyug, Andrey and Pamula, Elzbieta (2018) Pectin coatings on titanium alloy scaffolds produced by additive manufacturing:Promotion of human bone marrow stromal cell proliferation. Materials Letters, 227. pp. 225-228. ISSN 0167-577X

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Abstract

Ti6Al4V is a popular biomaterial for load-bearing implants for bone contact, which can be fabricated by additive manufacturing technologies. Their long-term success depends on their stable anchoring in surrounding bone, which in turn depends on formation of new bone tissue on the implant surface, for which adhesion and proliferation of bone-forming cells is a pre-requisite. Hence, surface coatings which promote cell adhesion and proliferation are desirable. Here, Ti6Al4V discs prepared by additive manufacturing (EBM) were coated with layers of pectins, calcium-binding polysaccharides derived from citrus (C) and apple (A), which also contained alkaline phosphatase (ALP), the enzyme responsible for mineralization of bone tissue. Adhesion and proliferation of human bone marrow stromal cells (hBMSC) were assessed. Proliferation after 7 days was increased by A-ALP coatings and, in particular, by C-ALP coatings. Cell morphology was similar on coated and uncoated samples. In conclusion, ALP-loaded pectin coatings promote hBMSC adhesion and proliferation.

Item Type: Journal Article
Journal or Publication Title: Materials Letters
Additional Information: This is the author’s version of a work that was accepted for publication in Materials Letters. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Materials Letters, 227, 2018 DOI: 10.1016/j.matlet.2018.05.060
Uncontrolled Keywords: /dk/atira/pure/subjectarea/asjc/3100/3104
Subjects:
Departments: Faculty of Science and Technology > Engineering
ID Code: 125122
Deposited By: ep_importer_pure
Deposited On: 14 May 2018 08:42
Refereed?: Yes
Published?: Published
Last Modified: 23 Feb 2020 04:27
URI: https://eprints.lancs.ac.uk/id/eprint/125122

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