Genomic and biological characterization of Newcastle disease viruses isolated from migratory mallards (Anas platyrhynchos)

Habib, Momena and Yaqub, Tahir and Nazir, Jawad and Shehzad, Wasim and Aziz-Ul-Rahman, and Sohail, Tayyebah and Mukhtar, Nadia and Mehboob, Arsalan and Munir, Muhammad and Shabbir, Muhammad Zubair (2018) Genomic and biological characterization of Newcastle disease viruses isolated from migratory mallards (Anas platyrhynchos). Archives of Virology, 163 (8). pp. 2179-2188. ISSN 0304-8608

[img]
Preview
PDF (Manuscript for LU)
Manuscript_for_LU.pdf - Accepted Version
Available under License Creative Commons Attribution-NonCommercial.

Download (1MB)

Abstract

Given the global evolutionary dynamics of Newcastle disease viruses (NDVs), it is imperative to continue extensive surveillance, routine monitoring and characterization of isolates originating from natural reservoirs (waterfowls). In this report, we isolated and characterized two virulent NDV strains from clinically healthy mallard (Anas platyrhynchos). Both isolates had a genome of 15,192 nucleotides encoding six genes in an order of 3´-NP-P-M-F-HN-L-5´. The biological characteristics (mean death time: 49.5-50 hr, EID50108.5 ml-1) and presence of a typical cleavage site in the fusion (F) protein (112R-R-Q-K-R↓F117) confirmed the velogenic nature of these isolates. Phylogenetic analysis classified both isolates as members of genotype VII within class-II. Furthermore, based upon the hypervariable region of the F gene (375 nt), isolates showed clustering within sub-genotype VIIi. Similarity index and parallel comparison revealed a higher nucleotide divergence from commonly used vaccine strains; LaSota (21%) and Mukteswar (17%). A comparative residues analysis with representative strains of different genotypes, including vaccine strains, revealed a number of substitutions at important structural and functional domains within the F and hemagglutinin-neuraminidase (HN) proteins. Together, the results highlight consistent evolution among circulating NDVs supporting extensive surveillance of the virus in waterfowl to better elucidate epidemiology, evolutionary relationships and their impacts on commercial and backyard poultry.

Item Type: Journal Article
Journal or Publication Title: Archives of Virology
Additional Information: The final publication is available at Springer via http://dx.doi.org/10.1007/s00705-018-3840-8
Uncontrolled Keywords: /dk/atira/pure/subjectarea/asjc/2400/2406
Subjects:
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 125061
Deposited By: ep_importer_pure
Deposited On: 23 May 2018 09:56
Refereed?: Yes
Published?: Published
Last Modified: 22 Feb 2020 04:32
URI: https://eprints.lancs.ac.uk/id/eprint/125061

Actions (login required)

View Item View Item