Porous calcium phosphate glass microspheres for orthobiologic applications

Hossain, Kazi Md Zakir and Patel, Uresha and Kennedy, Andrew and Macri-Pellizzeri, Laura and Sottile, Virginie and Grant, David M. and Scammell, Brigitte E. and Ahmed, Ifty (2018) Porous calcium phosphate glass microspheres for orthobiologic applications. Acta Biomaterialia, 72. pp. 396-406. ISSN 1742-7061

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Abstract

Orthobiologics is a rapidly advancing field utilising cell-based therapies and biomaterials to enable the body to repair and regenerate musculoskeletal tissues. This paper reports on a cost-effective flame spheroidisation process for production of novel porous glass microspheres from calcium phosphate-based glasses to encapsulate and deliver stem cells. Careful selection of the glass and pore-forming agent, along with a manufacturing method with the required processing window enabled the production of porous glass microspheres via a single-stage manufacturing process. The morphological and physical characterisation revealed porous microspheres with tailored surface and interconnected porosity (up to 76 ± 5%) with average pore size of 55 ± 8 µm and surface areas ranging from 0.34 to 0.9 m2 g−1. Furthermore, simple alteration of the processing parameters produced microspheres with alternate unique morphologies, such as with solid cores and surface porosity only. The tuneable porosity enabled control over their surface area, degradation profiles and hence ion release rates. Furthermore, cytocompatibility of the microspheres was assessed using human mesenchymal stem cells via direct cell culture experiments and analysis confirmed that they had migrated to within the centre of the microspheres. The novel microspheres developed have huge potential for tissue engineering and regenerative medicine applications. Statement of Significance This manuscript highlights a simple cost-effective one-step process for manufacturing porous calcium phosphate-based glass microspheres with varying control over surface pores and fully interconnected porosity via a flame spheroidisation process. Moreover, a simple alteration of the processing parameters can produce microspheres which have a solid core with surface pores only. The tuneable porosity enabled control over their surface area, degradation profiles and hence ion release rates. The paper also shows that stem cells not only attach and proliferate but more importantly migrate to within the core of the porous microspheres, highlighting applications for bone tissue engineering and regenerative medicine.

Item Type:
Journal Article
Journal or Publication Title:
Acta Biomaterialia
Additional Information:
This is the author’s version of a work that was accepted for publication in Acta Biomaterialia. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Acta Biomaterialia, 72, 2018 DOI: 10.1016/j.actabio.2018.03.040
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1300/1305
Subjects:
ID Code:
125050
Deposited By:
Deposited On:
09 May 2018 12:44
Refereed?:
Yes
Published?:
Published
Last Modified:
29 Sep 2020 04:13