E. coli SbcCD and RecA control chromosomal rearrangement induced by an interrupted palindrome

Darmon, Elise and Eykelenboom, John K and Lincker, Frédéric and Jones, Lucy H and White, Martin and Okely, Ewa and Blackwood, John K and Leach, David R (2010) E. coli SbcCD and RecA control chromosomal rearrangement induced by an interrupted palindrome. Molecular Cell, 39 (1). pp. 59-70. ISSN 1097-2765

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Abstract

Survival and genome stability are critical characteristics of healthy cells. DNA palindromes pose a threat to genome stability and have been shown to participate in a reaction leading to the formation of inverted chromosome duplications centered around themselves. There is considerable interest in the mechanism of this rearrangement given its likely contribution to genome instability in cancer cells. This study shows that formation of large inverted chromosome duplications can be observed in the chromosome of Escherichia coli. They are formed at the site of a 246 bp interrupted DNA palindrome in the absence of the hairpin nuclease SbcCD and the recombination protein RecA. The genetic requirements for this spontaneous rearrangement are consistent with a pathway involving DNA degradation and hairpin formation, as opposed to a cruciform cleavage pathway. Accordingly, the formation of palindrome-dependent hairpin intermediates can be induced by an adjacent DNA double-stand break.

Item Type:
Journal Article
Journal or Publication Title:
Molecular Cell
Additional Information:
2010 Elsevier Inc. All rights reserved.
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1300/1312
Subjects:
ID Code:
124932
Deposited By:
Deposited On:
27 Apr 2018 13:20
Refereed?:
Yes
Published?:
Published
Last Modified:
01 Jan 2020 11:19