Molecular characterization of infectious bursal disease viruses from Pakistan

Shabbir, Muhammad Zubair and Ali, Muhammad and Abbas, Muhammad and Chaudhry, Umer Naveed and Zia-Ur-Rehman, and Munir, Muhammad (2016) Molecular characterization of infectious bursal disease viruses from Pakistan. Archives of Virology, 161 (7). pp. 2001-2006. ISSN 0304-8608

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Abstract

Since the first report of infectious bursal disease in Pakistan in 1987, outbreaks have been common even in vaccinated flocks. Despite appropriate administration of vaccines, concerns arise if the circulating strains are different from the ones used in the vaccine. Here, we sequenced the hypervariable region (HVR) of the VP2 gene of circulating strains of infectious bursal disease virus (IBDV) originating from outbreaks (n = 4) in broiler flocks in Pakistan. Nucleotide sequencing followed by phylogeny and deduced amino acid sequence analysis showed the circulating strains to be very virulent (vv) and identified characteristic residues at position 222 (A), 242 (I), 256 (I), 294 (I) and 299 (S). In addition, a substitution at positions 221 (Q→H) was found to be exclusive to Pakistani strains in our analysis, although a larger dataset is required to confirm this finding. Compared to vaccine strains that are commonly used in Pakistan, substitution mutations were found at key amino acid positions in VP2 that may be responsible for potential changes in neutralization epitopes and vaccine failure.

Item Type:
Journal Article
Journal or Publication Title:
Archives of Virology
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/2400/2406
Subjects:
ID Code:
124777
Deposited By:
Deposited On:
20 Apr 2018 15:32
Refereed?:
Yes
Published?:
Published
Last Modified:
17 Jun 2020 05:46