Isolating HeLa cell fractions enriched for clathrin-coated vesicles

Borner, Georg H H and Fielding, Andrew B. (2014) Isolating HeLa cell fractions enriched for clathrin-coated vesicles. Cold Spring Harbor Protocols, 2014 (11). pp. 1184-1187. ISSN 1940-3402

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HeLa cell lines can be experimentally manipulated using drugs or gene-silencing techniques such as RNA interference. Fractions enriched for clathrin-coated vesicles (CCVs) can be isolated from these cell lines and used to study the effects of these manipulations on the composition of CCVs. This protocol, originally developed in the laboratory of Margaret Robinson (Cambridge, United Kingdom), describes the preparation of a HeLa cell fraction that is enriched for a mixed population of CCVs and is suitable for analysis by mass spectroscopy, western blotting, or electron microscopy.

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Journal Article
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Cold Spring Harbor Protocols
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© 2014 Cold Spring Harbor Laboratory Press.
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03 Apr 2018 14:06
Last Modified:
15 Feb 2022 02:12