Isolating HeLa cell fractions enriched for clathrin-coated vesicles

Borner, Georg H H and Fielding, Andrew B. (2014) Isolating HeLa cell fractions enriched for clathrin-coated vesicles. Cold Spring Harbor Protocols, 2014 (11). pp. 1184-1187. ISSN 1940-3402

Full text not available from this repository.

Abstract

HeLa cell lines can be experimentally manipulated using drugs or gene-silencing techniques such as RNA interference. Fractions enriched for clathrin-coated vesicles (CCVs) can be isolated from these cell lines and used to study the effects of these manipulations on the composition of CCVs. This protocol, originally developed in the laboratory of Margaret Robinson (Cambridge, United Kingdom), describes the preparation of a HeLa cell fraction that is enriched for a mixed population of CCVs and is suitable for analysis by mass spectroscopy, western blotting, or electron microscopy.

Item Type:
Journal Article
Journal or Publication Title:
Cold Spring Harbor Protocols
Additional Information:
© 2014 Cold Spring Harbor Laboratory Press.
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1300
Subjects:
ID Code:
124352
Deposited By:
Deposited On:
03 Apr 2018 14:06
Refereed?:
Yes
Published?:
Published
Last Modified:
08 Jul 2020 07:10