Using in-solution digestion, peptide fractionation, and a Q exactive mass spectrometer to analyze the proteome of clathrin-coated vesicles

Borner, Georg H H and Fielding, Andrew B. (2014) Using in-solution digestion, peptide fractionation, and a Q exactive mass spectrometer to analyze the proteome of clathrin-coated vesicles. Cold Spring Harbor Protocols, 2014 (11). pp. 1192-1195. ISSN 1940-3402

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Abstract

The characterization of clathrin-coated vesicles (CCVs), including the effects of genetic or biochemical manipulations on their composition, can be studied by mass spectrometry analysis of HeLa cell fractions enriched for CCVs. This protocol describes the preparation of samples by in-solution proteolytic digest and subsequent peptide fractionation, followed by analysis in a Q Exactive mass spectrometer.

Item Type:

Journal Article

Journal or Publication Title:

Cold Spring Harbor Protocols

Additional Information:

Uncontrolled Keywords:

/dk/atira/pure/subjectarea/asjc/1300/1300

Subjects:

?? cell fractionationclathrin-coated vesicleshela cellshumansmass spectrometryproteolysisproteomejournal articlegeneral biochemistry,genetics and molecular biologybiochemistry, genetics and molecular biology(all) ??

Departments:

Faculty of Health and Medicine > Biomedical & Life Sciences

ID Code:

124351

Deposited By:

ep_importer_pure

Deposited On:

03 Apr 2018 14:04

Refereed?:

Yes

Published?:

Published

Last Modified:

16 Jul 2024 10:40

URI:

https://eprints.lancs.ac.uk/id/eprint/124351