Using in-solution digestion, peptide fractionation, and a Q exactive mass spectrometer to analyze the proteome of clathrin-coated vesicles

Borner, Georg H H and Fielding, Andrew B. (2014) Using in-solution digestion, peptide fractionation, and a Q exactive mass spectrometer to analyze the proteome of clathrin-coated vesicles. Cold Spring Harbor Protocols, 2014 (11). pp. 1192-1195. ISSN 1940-3402

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Abstract

The characterization of clathrin-coated vesicles (CCVs), including the effects of genetic or biochemical manipulations on their composition, can be studied by mass spectrometry analysis of HeLa cell fractions enriched for CCVs. This protocol describes the preparation of samples by in-solution proteolytic digest and subsequent peptide fractionation, followed by analysis in a Q Exactive mass spectrometer.

Item Type:
Journal Article
Journal or Publication Title:
Cold Spring Harbor Protocols
Additional Information:
© 2014 Cold Spring Harbor Laboratory Press.
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1300
Subjects:
ID Code:
124351
Deposited By:
Deposited On:
03 Apr 2018 14:04
Refereed?:
Yes
Published?:
Published
Last Modified:
05 Aug 2020 06:05