Using in-gel digestion and an Orbitrap mass spectrometer to analyze the proteome of clathrin-coated vesicles

Borner, Georg H H and Fielding, Andrew B. (2014) Using in-gel digestion and an Orbitrap mass spectrometer to analyze the proteome of clathrin-coated vesicles. Cold Spring Harbor Protocols, 2014 (11). pp. 1188-1191. ISSN 1940-3402

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Abstract

The characterization of clathrin-coated vesicles (CCVs), including the effects of genetic or biochemical manipulations on their composition, can be studied by mass spectrometry analysis of HeLa cell fractions enriched for CCVs. This protocol describes the preparation of samples by tryptic in-gel digest and peptide extraction followed by analysis in an Orbitrap mass spectrometer.

Item Type:

Journal Article

Journal or Publication Title:

Cold Spring Harbor Protocols

Additional Information:

Uncontrolled Keywords:

/dk/atira/pure/subjectarea/asjc/1300/1300

Subjects:

?? cell fractionationclathrin-coated vesiclesgelshela cellshumansmass spectrometryproteolysisproteometrypsinjournal articlegeneral biochemistry,genetics and molecular biologybiochemistry, genetics and molecular biology(all) ??

Departments:

Faculty of Health and Medicine > Biomedical & Life Sciences

ID Code:

124350

Deposited By:

ep_importer_pure

Deposited On:

03 Apr 2018 14:04

Refereed?:

Yes

Published?:

Published

Last Modified:

16 Jul 2024 10:40

URI:

https://eprints.lancs.ac.uk/id/eprint/124350