Using in-gel digestion and an Orbitrap mass spectrometer to analyze the proteome of clathrin-coated vesicles

Borner, Georg H H and Fielding, Andrew B. (2014) Using in-gel digestion and an Orbitrap mass spectrometer to analyze the proteome of clathrin-coated vesicles. Cold Spring Harbor Protocols, 2014 (11). pp. 1188-1191. ISSN 1940-3402

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Abstract

The characterization of clathrin-coated vesicles (CCVs), including the effects of genetic or biochemical manipulations on their composition, can be studied by mass spectrometry analysis of HeLa cell fractions enriched for CCVs. This protocol describes the preparation of samples by tryptic in-gel digest and peptide extraction followed by analysis in an Orbitrap mass spectrometer.

Item Type:
Journal Article
Journal or Publication Title:
Cold Spring Harbor Protocols
Additional Information:
© 2014 Cold Spring Harbor Laboratory Press.
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1300/1300
Subjects:
?? cell fractionationclathrin-coated vesiclesgelshela cellshumansmass spectrometryproteolysisproteometrypsinjournal articlegeneral biochemistry,genetics and molecular biologybiochemistry, genetics and molecular biology(all) ??
ID Code:
124350
Deposited By:
Deposited On:
03 Apr 2018 14:04
Refereed?:
Yes
Published?:
Published
Last Modified:
16 Jul 2024 10:40