Isolation of clathrin-coated vesicles from tissue culture cells

Borner, Georg H H and Fielding, Andrew B. (2014) Isolation of clathrin-coated vesicles from tissue culture cells. Cold Spring Harbor Protocols, 2014 (11). pp. 1136-1138. ISSN 1940-3402

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Abstract

The study of clathrin-coated vesicles (CCVs) isolated from various organs has revealed the identities and important features of many of the factors involved in membrane trafficking. The development of isolation methods using cultured cell lines has made it possible to manipulate the source material before isolation to ask important questions about the roles of these factors and the pathways in which they are involved. We discuss here the advantages and limitations of the use of cultured cell lines for the isolation of CCVs.

Item Type:
Journal Article
Journal or Publication Title:
Cold Spring Harbor Protocols
Additional Information:
© 2014 Cold Spring Harbor Laboratory Press.
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1300
Subjects:
ID Code:
124349
Deposited By:
Deposited On:
03 Apr 2018 14:02
Refereed?:
Yes
Published?:
Published
Last Modified:
01 Jan 2020 11:16