Isolation of clathrin-coated vesicles from tissue culture cells

Borner, Georg H H and Fielding, Andrew B. (2014) Isolation of clathrin-coated vesicles from tissue culture cells. Cold Spring Harbor Protocols, 2014 (11). pp. 1136-1138. ISSN 1940-3402

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The study of clathrin-coated vesicles (CCVs) isolated from various organs has revealed the identities and important features of many of the factors involved in membrane trafficking. The development of isolation methods using cultured cell lines has made it possible to manipulate the source material before isolation to ask important questions about the roles of these factors and the pathways in which they are involved. We discuss here the advantages and limitations of the use of cultured cell lines for the isolation of CCVs.

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Journal Article
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Cold Spring Harbor Protocols
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© 2014 Cold Spring Harbor Laboratory Press.
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03 Apr 2018 14:02
Last Modified:
22 Nov 2022 05:40