Integrin-linked kinase localizes to the centrosome and regulates mitotic spindle organization

Fielding, Andrew B. and Dobreva, Iveta and McDonald, Paul C and Foster, Leonard J and Dedhar, Shoukat (2008) Integrin-linked kinase localizes to the centrosome and regulates mitotic spindle organization. Journal of Cell Biology, 180 (4). pp. 681-9. ISSN 0021-9525

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Abstract

Integrin-linked kinase (ILK) is a serine-threonine kinase and scaffold protein with well defined roles in focal adhesions in integrin-mediated cell adhesion, spreading, migration, and signaling. Using mass spectrometry-based proteomic approaches, we identify centrosomal and mitotic spindle proteins as interactors of ILK. alpha- and beta-tubulin, ch-TOG (XMAP215), and RUVBL1 associate with ILK and colocalize with it to mitotic centrosomes. Inhibition of ILK activity or expression induces profound apoptosis-independent defects in the organization of the mitotic spindle and DNA segregation. ILK fails to localize to the centrosomes of abnormal spindles in RUVBL1-depleted cells. Additionally, depletion of ILK expression or inhibition of its activity inhibits Aurora A-TACC3/ch-TOG interactions, which are essential for spindle pole organization and mitosis. These data demonstrate a critical and unexpected function for ILK in the organization of centrosomal protein complexes during mitotic spindle assembly and DNA segregation.

Item Type:
Journal Article
Journal or Publication Title:
Journal of Cell Biology
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1300/1307
Subjects:
ID Code:
124348
Deposited By:
Deposited On:
03 Apr 2018 13:58
Refereed?:
Yes
Published?:
Published
Last Modified:
24 Jun 2020 05:14