Identification of Leaf Promoters for Use in Transgenic Wheat

Alotaibi, Saqer and Sparks, Caroline and Parry, Martin and Simkin, Andrew and Raines, Christine (2018) Identification of Leaf Promoters for Use in Transgenic Wheat. Plants, 7 (2). ISSN 2223-7747

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Abstract

Wheat yields have plateaued in recent years and given the growing global population there is a pressing need to develop higher yielding varieties to meet future demand. Genetic manipulation of photosynthesis in elite wheat varieties offers the opportunity to significantly increase yields. However, the absence of a well-defined molecular tool-box of promoters to manipulate leaf processes in wheat hinders advancements in this area. Two promoters, one driving the expression of sedoheptulose-1,7-bisphosphatase (SBPase) and the other fructose-1,6-bisphosphate aldolase (FBPA) from Brachypodium distachyon were identified and cloned into a vector in front of the GUS reporter gene. Both promoters were shown to be functionally active in wheat in both transient assays and in stably transformed wheat plants. Analysis of the stable transformants of wheat (cv. Cadenza) showed that both promoters controlled gus expression throughout leaf development as well as in other green tissues. The availability of these promoters provides new tools for the expression of genes in transgenic wheat leaves and also paves the way for multigene manipulation of photosynthesis to improve yields.

Item Type:
Journal Article
Journal or Publication Title:
Plants
Subjects:
ID Code:
124322
Deposited By:
Deposited On:
03 Apr 2018 09:16
Refereed?:
Yes
Published?:
Published
Last Modified:
08 Jul 2020 07:10