Electrophysiological and pharmacological characteristics of buccal smooth muscle of the pest slug Deroceras reticulatum.

Wright, T. J. and Huddart, H. (1999) Electrophysiological and pharmacological characteristics of buccal smooth muscle of the pest slug Deroceras reticulatum. Journal of Comparative Physiology B, 169 (8). pp. 561-568. ISSN 0174-1578

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Abstract

Buccal mass muscle of the pest slug Deroceras reticulatum was examined by conventional tension recording and the sucrose-gap electrophysiological technique. Elevated potassium salines induced dose-dependent depolarisations accompanied by tonic contractures with superimposed rapid twitch contractions. The latter were suppressed at over 40 mmol · l−1 external potassium, where depolarisation-induced inactivation of voltage-sensitive calcium channels may have occurred. Acetylcholine caused significant dose-dependent depolarisations and tonic contractures, while 5-hydroxy tryptamine induced lower depolarisations accompanied by phasic contractile activity superimposed on low level tonic force. Of the purines examined only guanosine triphosphate caused significant mechanical activity above a threshold of 0.1 μmol · l−1. The tetrapeptides inhibited buccal muscle spontaneous activity, but the related small cardioactive peptide B was weakly excitatory. The amino acids glutamate and gamma-aminobutyric acid were weakly excitatory on buccal muscle while the molluscicides metaldehyde and methiocarb disrupted normal mechanical activity of the feeding musculature. Acetylcholine and 5-hydroxytryptamine appear to have major roles in regulating feeding muscle activity, seemingly modulated by guanosine triphosphate and inhibited by phenylalanine-methionine-arginine-phenylalanine-NH2 and phenylalanine-leucine-arginine-phenylalanine-NH2.

Item Type:
Journal Article
Journal or Publication Title:
Journal of Comparative Physiology B
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1300/1303
Subjects:
?? acetylcholine - deroceras reticulatum - fmrfamide - flrfamide - gtp 5-hydroxytryptamine - metaldehyde - methiocarb - sucrose gapbiochemistryecology, evolution, behavior and systematicsendocrinologyanimal science and zoologyphysiologyqh301 biology ??
ID Code:
10999
Deposited By:
Deposited On:
29 Jul 2008 09:14
Refereed?:
Yes
Published?:
Published
Last Modified:
15 Jul 2024 09:19