Regulation of Lysine Metabolism and Endosperm Protein Synthesis by the Opaque-5 and Opaque-7 Maize Mutations.

Azevedo, Ricardo A. and Lea, Peter John and Damerval, Catherine and Landry, Jacques and Bellato, Cláudia M. and Meinhardt, Lyndel W. and Le Guilloux, Martine and Delhaye, Sonia and Varisi, Vanderlei A and Gaziola, Salete A. and Gratão, Priscila Lupino and Toro, Alejandro A. (2004) Regulation of Lysine Metabolism and Endosperm Protein Synthesis by the Opaque-5 and Opaque-7 Maize Mutations. Journal of Agricultural and Food Chemistry, 52 (15). pp. 4865-4871. ISSN 0021-8561

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Abstract

Two high lysine maize endosperm mutations, opaque-5 (o5) and opaque-7 (o7), were biochemically characterized for endosperm protein synthesis and lysine metabolism in immature seeds. Albumins, globulins, and glutelins, which have a high content of lysine, were shown to be increased in the mutants, whereas zeins, which contain trace concentrations of lysine, were reduced in relation to the wild-type lines B77xB79+ and B37+. These alterations in the storage protein fraction distribution possibly explain the increased concentration of lysine in the two mutants. Using two-dimensional polyacrylamide gel electrophoresis of proteins of mature grains, variable amounts of zein polypeptides were detected and considerable differences were noted between the four lines studied. The analysis of the enzymes involved in lysine metabolism indicated that both mutants have reduced lysine catabolism when compared to their respective wild types, thus allowing more lysine to be available for storage protein synthesis.

Item Type:
Journal Article
Journal or Publication Title:
Journal of Agricultural and Food Chemistry
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1100/1100
Subjects:
?? aspartate kinaselysinelysine 2-oxoglutarate reductasemaizestorage proteinsgeneral agricultural and biological sciencesgeneral chemistryagricultural and biological sciences(all)chemistry(all)qh301 biology ??
ID Code:
10662
Deposited By:
Deposited On:
22 Jul 2008 08:21
Refereed?:
Yes
Published?:
Published
Last Modified:
16 Jul 2024 08:08